Re: Opening .btf files

Posted by Johannes-P. Koch on Nov 27, 2009; 10:30am
URL: http://imagej.273.s1.nabble.com/Opening-btf-files-tp3690296p3690297.html

Dear Colin,

I have the same problem; First, I have to mention that the handling of
big files is not sophisticated in the Olympus software. I am in contact
with Olympus, they are currently working on that. Specifially, I
suppose, that it should be possible to save big files (>2gb) in tif.
Now, the program simply does not allow this action.

What you need to open such files in ImageJ is the LOCI Bioformats
plugin; works perfectly. An alternative would be to not save your files
as bigtiff, but as split tifs and import the series in ImageJ via
File->Import->Image Sequence.

Best,
Johannes

Colin Rickman schrieb:

> Hi
>
> I have acquired some large 4D widefield datasets using an Olympus
> microscope running the CellR software. The files are very large and
> have been saved in a .btf file format. I have the most stable version
> of bioformats installed but can't open them using imagej. Is this file
> format supproted in any way by imagej?
>
> Hope someone can help
>
> Regards
>
> Colin
>
> --
> Dr Colin Rickman
> School of Engineering and Physical Sciences Heriot-Watt University
> Edinburgh
> EH14 4AS
>
> Tel: [phone]
> Fax: [phone]
>

--
Mag. Johannes-P. KOCH
Department of Biochemistry and Cell Biology
MFPL, University of Vienna
Dr. Bohrgasse 9/5
A-1030 Vienna
Austria

phone: [phone]
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