Re: Opening .btf files

Posted by Colin Rickman-3 on Nov 27, 2009; 10:36am
URL: http://imagej.273.s1.nabble.com/Opening-btf-files-tp3690296p3690298.html

Dear Johannes

Thanks for getting in touch. I don't think it is possible to save such
large files as a .tif file given the inherent limit on file size. I
think that is where the big tiff (.btf) format is preferable. As I said
in my message I have tried the most recent version of Bioformats but it
cannot open the file. Maybe the way CellR wites the .btf file is
incompatible. Which version of CellR are you using?

At the moment I am exporting the data using CellR to individual Z-stack
.tif files to restitch together.

Colin

Johannes-P. Koch wrote:

> Dear Colin,
>
> I have the same problem; First, I have to mention that the handling of
> big files is not sophisticated in the Olympus software. I am in
> contact with Olympus, they are currently working on that. Specifially,
> I suppose, that it should be possible to save big files (>2gb) in tif.
> Now, the program simply does not allow this action.
>
> What you need to open such files in ImageJ is the LOCI Bioformats
> plugin; works perfectly. An alternative would be to not save your
> files as bigtiff, but as split tifs and import the series in ImageJ
> via File->Import->Image Sequence.
>
> Best,
> Johannes
>
> Colin Rickman schrieb:
>> Hi
>>
>> I have acquired some large 4D widefield datasets using an Olympus
>> microscope running the CellR software. The files are very large and
>> have been saved in a .btf file format. I have the most stable version
>> of bioformats installed but can't open them using imagej. Is this
>> file format supproted in any way by imagej?
>>
>> Hope someone can help
>>
>> Regards
>>
>> Colin
>>
>> --
>> Dr Colin Rickman
>> School of Engineering and Physical Sciences Heriot-Watt University
>> Edinburgh
>> EH14 4AS
>>
>> Tel: [phone]
>> Fax: [phone]
>>
>
>

--
--
Dr Colin Rickman
School of Engineering and Physical Sciences Heriot-Watt University Edinburgh
EH14 4AS

Tel: [phone]
Fax: [phone]