Re: Particle Analysis

Posted by Stoyan Pavlov on
URL: http://imagej.273.s1.nabble.com/Particle-Analysis-tp3690320p3690324.html

Hi Florian,
Clicking twice on the magic wand icon brings up a window for configuration.

Best regards

Stoyan Pavlov
On Thu, Nov 26, 2009 at 1:17 PM, Florian Sedlmeir
<[hidden email]> wrote:

> Hi,
>
> I want to analyse particle-like structures with ImageJ. The problem is, that
> these particles usually have a good contrast against their local
> environment, but globally there are too many areas with similar or same grey
> values to threshold the image. So i have a few questions concerning this:
>
> - Is there an easy way to threshold only selected areas in a picture (to
> threshold the whole picture step by step)?
>
> - My particles are easily distinguishable with the eye because they are not
> as rough as their environment (+usually  there is a local difference in
> contrast). ImageJ is able to find the edges with the "find edges" tool, but
> unfortunatly it doesn´t create closed areas (my plan was to find edges,
> threshold the resulting picture and analyse the resulting particles then) Is
> there a solution for this a problem?
>
> - One of my friends showed me the "magic wand" tool in Photoshop, which is
> able to determine my particles properly. Unfortunatly the same tool in
> ImageJ is not able to do this, it seems to be too sensitive. (Are there
> parameters which can be adjustet?)
> So my question is: Does a plugin exist, which is able to do the same task as
> Photoshop´s magic wand?
>
>
> Thank you and best regards.
> Florian
>

--
Dr. Stoyan P. Pavlov, MD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
 Bulgaria
Tel: +359 (0) 52 - 677 - 050 #2638
e-mail: [hidden email]