Re: Cell counting with imageJ

Posted by G. Esteban Fernandez on
URL: http://imagej.273.s1.nabble.com/Cell-counting-with-imageJ-tp3690375p3690379.html

How many images/cells do you need to count?  Is this something that
you can do by hand in a matter of a couple of days or do you want an
automated procedure?  Often it takes a few days to get an automated
procedure to work correctly, if it ever works at all, during which
time you could've done the counting by hand.  There are probably
ImageJ plugins that keep track of counts as you click on cells in the
images.

Do you have images that match the fluorescence images to get total
cell numbers?  For example transmitted light (brightfield/phase/DIC)
images?

-Esteban


On Fri, Nov 13, 2009 at 7:43 AM, Antonis Giakountis <[hidden email]> wrote:

> Dear all,
>
> I am a plant Biologist and I would like to use ImageJ in order to count cell numbers (diameter 10-20 microns on average) and to estimate percentage of cells that have fluorescence.
>
> Is it possible to to that with ImageJ and if yes do I have to install a certain plugin? If not can you recomment another software?
>
> Thank you in advance for your time,
>
> Antonis
>
> --------------------------------------------
> Antonis Giakountis, PhD
> Prof. David Baulcombe group
> Department of Plant Sciences
> University of Cambridge
> Downing Street
> Cambridge CB2 3EA
> UK
>

--
G. Esteban Fernandez, Ph.D.

Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO  65211

http://www.biotech.missouri.edu/mcc

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