Re: Cell counting with imageJ

Posted by Antonis Giakountis on
URL: http://imagej.273.s1.nabble.com/Cell-counting-with-imageJ-tp3690375p3690387.html

Thanks Damon, I am doing an immunofluorescence as we speak. If I get it to
work nicely I will attach an image and send it over. I expect my
fluorescence to be in the nucleus (approx. 1-2 microns) of a rather small
cell size population (10-15 microns). I must admit that I haven't played
with imageJ before so I will have a look at the documentation as well.

Cheers,

Antonis
----- Original Message -----
From: "Damon Poburko" <[hidden email]>
To: <[hidden email]>
Sent: Friday, November 13, 2009 2:04 PM
Subject: Re: Cell counting with imageJ

> Dear Antonis,
>
>  Image J is definitely suited to counting cells. How you go about doing so
> depends entirely on what your images look like and how you plan to
> identify cells that are fluorescent and non-fluorescent. You'll have to
> provide more details of what your images look like. Also have a read
> through the Image J documentation if you haven't done so already. I think
> there are some good hints to getting started in there.
>
> Cheers,
> Damon
>
>
> Antonis Giakountis wrote:
>> Dear all,
>>
>> I am a plant Biologist and I would like to use ImageJ in order to count
>> cell numbers (diameter 10-20 microns on average) and to estimate
>> percentage of cells that have fluorescence.
>>
>> Is it possible to to that with ImageJ and if yes do I have to install a
>> certain plugin? If not can you recomment another software?
>>
>> Thank you in advance for your time,
>>
>> Antonis
>>
>> --------------------------------------------
>> Antonis Giakountis, PhD
>> Prof. David Baulcombe group
>> Department of Plant Sciences
>> University of Cambridge
>> Downing Street
>> Cambridge CB2 3EA
>> UK
>>
>