Re: flattening a layer from z-stacks of thick uneven tissue

Posted by Gabriel Landini on Oct 13, 2009; 2:22pm
URL: http://imagej.273.s1.nabble.com/flattening-a-layer-from-z-stacks-of-thick-uneven-tissue-tp3690504p3690515.html

On Tuesday 13 October 2009  15:21:14 Mark Krebs wrote:
> My images are of a transparent object with fluorescent nuclei in many
> layers.

Sorry to ask the obvious. Have you tried thresholding the stack?
Cheers,
G.