Re: Question about image stacking problem

Posted by JeremyR1 on
URL: http://imagej.273.s1.nabble.com/Question-about-image-stacking-problem-tp3690530p3690532.html

Thanks very much! I really appreciate it.

Jeremy

Wayne Rasband wrote

The Image>Stacks>Images to Stacks command does not work properly with  
8-bit color image. This will be fixed in the next daily build. In the  
meantime, you can work around the problem by converting each of the  
images to RGB (Image>Type>RGB Color) before using the Images to Stack  
command.

-wayne

On Nov 7, 2009, at 8:19 AM, JeremyR1 wrote:

 > Hi, I'm new to ImageJ.
 >
 > I searched through the forum, but couldn't find an answer to
 > this issue: I'm trying to simply stack images. The images
 > are all the same size, 8-bit tif files. Anytime I stack
 > images, the second image and all following images in the
 > stack look different than their original source image - they
 > have a more pixelated look than their original source image.
 > It looks almost as if some thresholding or contrasting
 > command is being automatically performed. The first image of
 > the stack looks unchanged compared to its original source
 > image.
 >
 > Oddly enough, if I stack a couple copies of the same image,
 > this doesn't happen. I tried resetting all the image
 > adjusting parameters of the stacked image (i.e. reset
 > threshold, reset brightness/contrast, etc), but that didn't
 > make the stacked image look closer to the source image.
 >
 > Has anyone encountered this, and know how to fix it?
 >
 > I'm using ImageJ 1.42q, running on a Mac with OSX 10.5.8.