Re: Question about image stacking problem

Posted by John Oreopoulos on Nov 07, 2009; 4:10pm
URL: http://imagej.273.s1.nabble.com/Question-about-image-stacking-problem-tp3690530p3690533.html

Jeremy,

When you stack images, they are all forced into the same brightness  
and contrast settings of the first image in the stack.

John Oreopoulos

On 7-Nov-09, at 8:19 AM, JeremyR1 wrote:

> Hi,
> I'm new to ImageJ.
>
> I searched through the forum, but couldn't find an answer to this  
> issue:
> I'm trying to simply stack images. The images are all the same  
> size, 8-bit
> tif files.
> Anytime I stack images, the second image and all following images  
> in the
> stack look different than their original source image - they have a  
> more
> pixelated look than their original source image. It looks almost as  
> if some
> thresholding or contrasting command is being automatically  
> performed. The
> first image of the stack looks unchanged compared to its original  
> source
> image.
>
> Oddly enough, if I stack a couple copies of the same image, this  
> doesn't
> happen.
> I tried resetting all the image adjusting parameters of the stacked  
> image
> (i.e. reset threshold, reset brightness/contrast, etc), but that  
> didn't make
> the stacked image look closer to the source image.
>
> Has anyone encountered this, and know how to fix it?
>
> I'm using ImageJ 1.42q, running on a Mac with OSX 10.5.8.
>
> Thanks very much in advance,
> Jeremy
> --
> View this message in context: http://n2.nabble.com/Question-about- 
> image-stacking-problem-tp3964128p3964128.html
> Sent from the ImageJ mailing list archive at Nabble.com.