FRAP: measure intensity of moving foci problem. Is there a moving ROI application in ImageJ
Posted by MartijndeG on Oct 29, 2009; 2:16pm
URL: http://imagej.273.s1.nabble.com/FRAP-measure-intensity-of-moving-foci-problem-Is-there-a-moving-ROI-application-in-ImageJ-tp3690606.html
Hello everybody,
I'm working with ImageJ for some time and I need a certain function/macro/plugin for my research.
I bleach certain parts of foci with "FRAP on the Fly" and when I analyse the image stack (.stk MetaMorph), I form a ROI over the bleached region and measure the intensity during the recovery of the fluorescence signal.
The problem is that these foci are moving, not much, but in the period of measurement they migrate their own size in distance, so measuring this bleached region with a ROI results in lost of signal due the migration out of the ROI.
Is there a solution for this problem, I can understand this happens with more FRAP experiments?
I thought myself there must be an application to let the ROI move over a certain predefined distance, to cover the bleached region.
many thanks in advance!