http://imagej.273.s1.nabble.com/Meassure-pupil-diameter-from-video-file-tp3690637p3690638.html
sample of your data.
> Hi!
> I reseached a litle bit about it but couldn't find a macro to suit my needs
> and I'm not familiar to Java programming (well, in fact, to programing, at
> all).
>
>
> I plan to record pupilar constriction during 2-5 minutes upon light pulses.
> The idea is to find a macro or plugin that allows me to measure the pupilar
> radius or diameter or area.
>
> So, I suppose te macro should:
> -Allow me to convert a video recording into frames. (I suppose this can be
> done via virtualdub, but I'm not so sure that imageJ would be able to
> understand it).
> -Find two concentrical circles (pupil and iris) and meassure the one inside
> or the distance between both.
> -It should be able to write the pupilar values along with the corresponding
> time or frame values in a file.
> -The tool should be able to measure from color images obtained in standard
> light and infrared conditions(CCTV camera, not sure jet if it is black and
> white or green, but I suppose it could be converted easily).
>
> I know, I may be asking for to much. I pretend to use it in a publication
> about the correspondence of this pupilar constriction and a protein highly
> responsible of this activity, melanopsin. Having this tool would mean tons
> of flexibilty and saving numerous animal lifes.
> If somebody wants to join I can only offer appearing in the final
> publication.
>
> Thanks for your time and if someone wants to help, you are highly welcome:
>
[hidden email]
>