Re: unrolling a z-stack

Posted by Bill Mohler on Oct 15, 2009; 6:38pm
URL: http://imagej.273.s1.nabble.com/unrolling-a-z-stack-tp3690733p3690735.html

I would suggest rotating projections around the y-axis
(Image>Stack>3dProject), followed by cropping to a small central region
for each projection image, and then use of some sort of stitching
software to build the whole "panorama".

There is a whole field of panoramic photographic rendering that also has
listservs that might help with the stitching details.  There may also be
ImageJ plugins to do the work.

Elizabeth Crowell wrote:

> Dear ImageJ experts,
>
> I am taking images of fibers that occur on the periphery of cells, and acquiring
> z-stacks of the entire cell.  The cells are roughly cylindrical.
> For visualization purposes, it would be useful to virtually slice open these
> cells and unroll them so that I can see the fibers on every face of the
> cylinder in the same plane.
> Is there a means of doing this?  Has anyone encountered this issue before?
>
> Segmentation of the cells should not be a problem, since I can simply delete the
> signal outside of the cells in each slice.  (This does not need to be a
> high-through-put procedure.)
>
> Thank you in advance for your ideas...
>
> Sincerely,
>
> Elizabeth Crowell
> Laboratoire de Biologie Cellulaire
> INRA / Institut Jean-Pierre Bourgin
> Route de Saint-Cyr
> 78026 Versailles cedex
> FRANCE
>
> Tel: [phone]
> Fax: [phone]
>
>  

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William A. Mohler
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