Re: quick way to select the maximum pixel
Posted by
Gabriel Landini on
Oct 08, 2009; 8:28am
URL: http://imagej.273.s1.nabble.com/quick-way-to-select-the-maximum-pixel-tp3690812p3690816.html
> We recently discovered a couple of dead pixels in our monochrome camera.
> They only show up when people have very very low intensity labelling and
> are using long exposure times so normally not a problem.
>
> However, it was suggested to me that to eliminate them from the images,
> I could "find" the dead pixels (since they are maximum intensity) and
> then fill them with the average grayscale value of the background (I
> guess the minimum?).
>
> Can anyone offer me some suggestions on how to do this?
Have a look at the macro here. It will convert all the single pixels which
have a value of 255 to the mean of the neighbours (colour or greyscale image).
http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopyI hope it helps
Gabriel