Re: scratch wound assay

Posted by Pullikuth, Ashok K. on Sep 21, 2009; 3:50pm
URL: http://imagej.273.s1.nabble.com/Saving-ROI-tp3691075p3691081.html

I came across a software called TScratch for automated analysis of monolayer wound healing assays. The paper describing this was recently published in Biotechniques:

Geback et al., 2009. TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays. Biotechniques 46(4) 265-274.

The stand alone version is available from www.cse-lab.ethz.ch/software.html

_________________________________________
Ashok Pullikuth PhD





-----Original Message-----
From: ImageJ Interest Group on behalf of Pascal Lorentz
Sent: Mon 9/21/2009 10:15 AM
To: [hidden email]
Subject: scratch wound assay
 
Dear all,

I was wondering, what is the best way the measure scratches in a wound
assay.
So far I just used the polygon selection to line out the cap between the
cells and took the area as a measurement.
Is there a better way to do it? Maybe a plugin or any other automated
procedure?
Any help is appreciated.

Best regards

Pascal

Pascal Lorentz
Department of Biomedicine
University of Basel
Mattenstrasse 28
4058 Basel
Switzerland