Re: Advice request: Recognizing and measuring diseased leaf areas

Posted by Jean-Yves Tinevez-2 on
URL: http://imagej.273.s1.nabble.com/Advice-request-Recognizing-and-measuring-diseased-leaf-areas-tp3691171p3691172.html

On Sep 15, 2009, at 8:56 AM, Jonathan Rosenthal wrote:

> I'd greatly appreciate any suggestions as to how I can use ImageJ on  
> scanned, color images of leaves to detect several different plant  
> diseases and to measure the areas affected (they turned yellow or  
> brown or grew small bumps on the surface).
>    Thanks very much,

Can you put an image somewhere?
 From what you say it concerns Color-based image segmentation, so I  
would try to use Color Deconvolution:

http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

It is an awesome plugin that can separate your image according to  
certain color, initially meant to deal with immunostaining. It would  
allow you to separate the image of te leave from the brown bumps, that  
you could then process separately.
Your image should be relatively flat.

Cheers
jy



--
Jean-Yves Tinevez
PFID - Imagopole
Institut Pasteur
25-28, rue du Docteur Roux
75724 Paris cedex 15
France
tel: [phone]