Re: Different results with duplicate or re-open the image

Posted by William Joseph Ashby on Aug 26, 2009; 2:18pm
URL: http://imagej.273.s1.nabble.com/Different-results-with-duplicate-or-re-open-the-image-tp3691380p3691386.html

I've noticed that the duplicate command will only duplicate the region of
interest if you have selected a portion of the image.  That might cause your
problem.

-Will Ashby


On 8/26/09 9:06 AM, "Karel Jansseune" <[hidden email]>
wrote:

> Hello imageJ users,
>
> I have a strange problem with my macro.
> The macro is used to measure a certain part of the image that contains
> plant parts.
>
> The macro is creating a mask of the image, this mask is then renamed
> as "mask", and this is used to filter out the parts that I need in the
> next measuring step by subtracting it from the original image.
>
> In the first version I used to re-open the original image and to apply the
> mask to that image.
> I have now discovered that if I duplicate the image in stead of re-opening
> it, I get different results when I measure the area of the plant parts.
> (up to 15% difference!).
>
> Is there a differnce between the original image and a duplicate in terms
> of resolution? or should I look for the error in another place?
>
> kind regards,
>
> Karel Jansseune