Re: Measurement of DNA with DAPI

Posted by Gabriel Landini on Aug 21, 2009; 7:27am
URL: http://imagej.273.s1.nabble.com/Measurement-of-DNA-with-DAPI-tp3691426p3691427.html

On Friday 21 August 2009, Manuel Bonilla wrote:
> I am trying to use Image J for measuring the amount of DNA in an algal
>  cell. How can this be done?

Have a look at this paper:

David C. Hardie, T. Ryan Gregory, and Paul D.N. Hebert, “From Pixels to
Picograms: A Beginners' Guide to Genome Quantification by Feulgen Image
Analysis Densitometry,” J. Histochem. Cytochem. 50, no. 6 (June 1, 2002):
735-750.

If I recall correctly the paper has a curious comment suggesting not doing
background correction. I am not sure that it is right, but the rest of the
paper is very informative.

G.