Re: Measurement of DNA with DAPI

Posted by dksamuel on Aug 21, 2009; 8:10am
URL: http://imagej.273.s1.nabble.com/Measurement-of-DNA-with-DAPI-tp3691426p3691428.html

Thanks for a nice paper, Samuel, Bangalore, India

On Fri, Aug 21, 2009 at 12:57 PM, Gabriel Landini<[hidden email]> wrote:

> On Friday 21 August 2009, Manuel Bonilla wrote:
>> I am trying to use Image J for measuring the amount of DNA in an algal
>>  cell. How can this be done?
>
> Have a look at this paper:
>
> David C. Hardie, T. Ryan Gregory, and Paul D.N. Hebert, “From Pixels to
> Picograms: A Beginners' Guide to Genome Quantification by Feulgen Image
> Analysis Densitometry,” J. Histochem. Cytochem. 50, no. 6 (June 1, 2002):
> 735-750.
>
> If I recall correctly the paper has a curious comment suggesting not doing
> background correction. I am not sure that it is right, but the rest of the
> paper is very informative.
>
> G.
>