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volume measurement and memory problems

Posted by Paola Lepanto on Jul 30, 2009; 10:40pm
URL: http://imagej.273.s1.nabble.com/volume-measurement-and-memory-problems-tp3691572.html

Hi everyone,
I´ve been stuck with some problems: regarding the computer memory
problems, JRockit JMV is no longer available to extend the memory that
ImageJ can use in 32bits Windows, anybody knows an alternative?
Regarding the volume measurement, I´m still trying to separate my
particle of interest from other stuff (a cell) in a confocal stack in
order to do particle analysis to calculate volume (the particle and the
cells have almost the same fluorescence intensity in the same channel
and are in contact in some regions). Watershed2D or 3D doesn´t work
beacause of under or oversegmentation as Johannes said. I´ve tryied out
other segmentation plugins but they don´t select what I want. May be
SegmentationAssistant plugin may work but it doesn´t retrieves any roi
and get stucked as soon as I try to change any parameter. The java that
is needed for this plugin (as is pointed out in the plugin webpage) is
no longer available, also. Is there any other alternative to my problem,
perhaps through 3Dreconstruction?.
Any idea will be very belcome, thanks
Paola