Blood vessel analysis using 16-bit image

Posted by Schaffer, Beverly on Jul 28, 2009; 6:05pm
URL: http://imagej.273.s1.nabble.com/Blood-vessel-analysis-using-16-bit-image-tp3691611.html

Hi Everyone,

 

Thanks to everyone who responded to my previous email about imaging
blood vessels. I've tried the skeletonize and analyze skeleton plug-ins,
but they only work with an 8-bit grayscale image. The camera I am using
takes a 16-bit picture. I have tried converting the image to 8-bit in
ImageJ (type-8-bit) and then processing the image using skeletonize. All
I get is a single straight line in the middle of the image.

 

I've tried saving the image in AxioVision as a tif and then taking it
into ImageJ, converting it to 8-bit (as above) with the same result when
I process using skeletonize. I've also tried taking the tif image into
photoshop and saving it as an 8-bit grayscale image and get the same
result when I process with skeletonize.

 

Any other ideas?

 

Thanks!

 

Beverly Schaffer

[hidden email]