Posted by
David Webster on
Jul 30, 2009; 6:54pm
URL: http://imagej.273.s1.nabble.com/Blood-vessel-analysis-using-16-bit-image-tp3691611p3691612.html
Beverly ,
I ran the user developed plugin "Skeletonize (2D/3D)" on the built in image
grayscale "blobs.gif". I got a straight line in the center of the image like
you did. So, if you were processing an 8-bit grayscale, try using
"Process/Binary/Make Binary" first.
David Webster
On Tue, Jul 28, 2009 at 11:05 AM, Schaffer, Beverly <
[hidden email]>wrote:
> Hi Everyone,
>
>
>
> Thanks to everyone who responded to my previous email about imaging
> blood vessels. I've tried the skeletonize and analyze skeleton plug-ins,
> but they only work with an 8-bit grayscale image. The camera I am using
> takes a 16-bit picture. I have tried converting the image to 8-bit in
> ImageJ (type-8-bit) and then processing the image using skeletonize. All
> I get is a single straight line in the middle of the image.
>
>
>
> I've tried saving the image in AxioVision as a tif and then taking it
> into ImageJ, converting it to 8-bit (as above) with the same result when
> I process using skeletonize. I've also tried taking the tif image into
> photoshop and saving it as an 8-bit grayscale image and get the same
> result when I process with skeletonize.
>
>
>
> Any other ideas?
>
>
>
> Thanks!
>
>
>
> Beverly Schaffer
>
>
[hidden email]
>