Re: Blood vessel analysis using 16-bit image

Posted by Gabriel Landini on Jul 28, 2009; 7:28pm
URL: http://imagej.273.s1.nabble.com/Blood-vessel-analysis-using-16-bit-image-tp3691611p3691613.html

On Tuesday 28 July 2009 19:05:37 Schaffer, Beverly wrote:
> I've tried the skeletonize and analyze skeleton plug-ins,
> but they only work with an 8-bit grayscale image. The camera I am using
> takes a 16-bit picture. I have tried converting the image to 8-bit in
> ImageJ (type-8-bit) and then processing the image using skeletonize. All
> I get is a single straight line in the middle of the image.

I guess that you are missing the step of segmenting/converting/thresholding
your 16 or 8 bit image into a binary image (which is also 8 bit).
After you have a binary image you skeletonise it.
(There are greyscale skeletons, but I do not think that is what you are
looking for).

Cheers

G.