Posted by
Schaffer, Beverly on
Jul 28, 2009; 8:21pm
URL: http://imagej.273.s1.nabble.com/Blood-vessel-analysis-using-16-bit-image-tp3691611p3691614.html
That sounds easy. Are those additional plug-ins (completely clueless) or
something available in ImageJ. I looked through all the drop down menus
and didn't find the options. I did find a threshold option under the
colocalization and a multi-threshold under the filter plug-in.
Could clarify for me?
Thanks!
Bev
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Gabriel Landini
Sent: Tuesday, July 28, 2009 2:28 PM
To:
[hidden email]
Subject: Re: Blood vessel analysis using 16-bit image
On Tuesday 28 July 2009 19:05:37 Schaffer, Beverly wrote:
> I've tried the skeletonize and analyze skeleton plug-ins,
> but they only work with an 8-bit grayscale image. The camera I am
using
> takes a 16-bit picture. I have tried converting the image to 8-bit in
> ImageJ (type-8-bit) and then processing the image using skeletonize.
All
> I get is a single straight line in the middle of the image.
I guess that you are missing the step of
segmenting/converting/thresholding
your 16 or 8 bit image into a binary image (which is also 8 bit).
After you have a binary image you skeletonise it.
(There are greyscale skeletons, but I do not think that is what you are
looking for).
Cheers
G.