Re: Blood vessel analysis using 16-bit image

Posted by Gabriel Landini on Jul 28, 2009; 9:05pm
URL: http://imagej.273.s1.nabble.com/Blood-vessel-analysis-using-16-bit-image-tp3691611p3691616.html

On Tuesday 28 July 2009 21:21:26 Schaffer, Beverly wrote:
> That sounds easy. Are those additional plug-ins (completely clueless) or
> something available in ImageJ. I looked through all the drop down menus
> and didn't find the options. I did find a threshold option under the
> colocalization and a multi-threshold under the filter plug-in.

I think (not sure what you did so far) you need to reduce the 16 or 8 bit
image of your blood vessels into a binary image that shows vessels in white
and background in black (or viceversa). That is a binary image (2 values: 0
and 255).
One method is via thresholding (Image>Adjust>Threshold). Depending what
version of the ij.jar file you've got, you might have 1 method or about 15.
However if that does not work, you might have to use a different approach
(like Laplacian of Gaussian [LoG] filter, or region based segmentation or
something different).
Once you have your binary image, call the "skeletonize" command. That will
thin the binary object to a skeleton.
Cheers,

Gabriel