RGB weights and fluorescence intensity

Posted by Sinead Roberts on
URL: http://imagej.273.s1.nabble.com/RGB-weights-and-fluorescence-intensity-tp3691651.html

Dear all,

I want to calculate the fluorescence intensity from an RGB image that I have thresholded. So to know
the total fluorescence intensity in a ROI am I right in thinking I can take the 'integrated density' value
in the measurements section? Also, should I calculate the brightness value with or without weighted
RGBs? What is the weighting for?

Thank you very much for your help, I am afraid I know very little about the principles of image
analysis and processing,

Sinead