http://imagej.273.s1.nabble.com/Counting-pixels-above-a-certain-grey-value-in-a-stack-tp3691747p3691752.html
16-bit image. As I understand it, that function works only with 8-bits.
--You might try changing the image type. It looks as if you don't need the
> Hi Jerome,
>
> Thank you for your helpful post. I am trying to do what you suggested, but
> I'm having a hard time getting the threshold function to work properly.
> When I access the threshold menu, this is what I get:
>
>
http://n2.nabble.com/file/n3269373/Picture%2B1.jpeg>
> For some reason, I am not able to set the value range for my image. Any
> advice would be much appreciated!
>
> Thanks,
>
> Barrett
>
>
>
>
> jerome mutterer-2 wrote:
> >
> > Hi.
> > I'm not sure a plugin is necessary in that case. Selecting pixel value
> > ranges is just exactly what the threshold does. I suggest the following
> > procedure :
> > * go to Image/Adjust/Threshold and set the value range you would like to
> > measure.
> > * go to Analyze/Set Measurements, and make sure you check the "limit to
> > threshold" checkbox and the "area" field.
> > * go to Image/Stacks/Plot Z-axis Profile
> > You're done.
> >
> > Jerome
> >
> >
> > On Dec 4, 2007 2:42 PM, Johannes Schindelin <
[hidden email]>
> > wrote:
> >
> >> Hi,
> >>
> >> On Tue, 4 Dec 2007, Bio7 wrote:
> >>
> >> > public class Count_Stack_Pixels implements PlugIn {
> >>
> >> If you implement a PlugInFilter (which returns DOES_8G | DOES_16 |
> >> NO_CHANGES in setup()) you do not need to find out the current image
> >> or check the image type yourself.
> >>
> >> > /*We create a dialog!*/
> >> > GenericDialog gd = new
> >> GenericDialog("Count Pixels");
> >> > gd.addNumericField("Min: ", min, 0);
> >> > gd.addNumericField("Max: ", max, 0);
> >>
> >> You could reuse the min/max setting from the threshold.
> >>
> >> Ciao,
> >> Dscho
> >>
> >
> >
>
> --
> View this message in context:
>
http://n2.nabble.com/Counting-pixels-above-a-certain-grey-value-in-a-stack-tp635323p3269373.html> Sent from the ImageJ mailing list archive at Nabble.com.
>
Joel B. Sheffield, Ph.D