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Re: more info - Vista/Bioimageformats

Posted by Robert Baer on Jul 08, 2009; 10:46pm
URL: http://imagej.273.s1.nabble.com/Stack-Problem-on-Vista-Bioimageformats-tp3691826p3691827.html

I tried Wayne's suggestion of updating LOCI using both today's daily build
and the 2008 stable build to no avail.

I have the following behavior that may provide the more knowledge with
insight.  If I load a .zvi  image stack into ImageJ with load into Image5D
selected instead of standard ImageJ selected I still get the scambled image
displayed. If I now switch from grayscale to ovl on the right the first
frame of the stack is displayed completely properly.  If I now move the
z-slider to a subsequent frame, I get a scrambled view of that frame.
Moving to color or grayscale and back to ovl make this subsequent frame
appear normally.  Finally, moving the slider back to frame1 produces a
scrambled view of Frame1.  After using the ovl trick to get a normal image,
performing other ImageJ operations such as Image->Adjust->Brightness and
contrast causes the frame to scramble.

Any additional insights.

Thanks,
Rob

----- Original Message -----
From: "Robert W. Baer, Ph.D." <[hidden email]>
To: <[hidden email]>
Sent: Wednesday, July 08, 2009 11:48 AM
Subject: Stack Problem on Vista/Bioimageformats


I have been experiencing some strange behavior when opening .zvi phase
z-stacks with ImageJ 1.43b on a Vista Machine.  The individual frames of the
stack have scrambled squares that each represent a small fraction of the
normal image, but with no particular order to the squares.  The squares
repeat maybe 4 times and don't together  represent the entire image.
Scrolling through the stack causes the frames to progress through the
z-plane focus, but each frame is made of scrambled squares.

Strangely, the (mis)behavior is not 100% consistent.  I can occasionaly get
the stacks to open like normal stacks.   I have superstiously attributed
this "normalcy" to running ImageJ update, opening as a hyperstack, rebooting
the computer, but in the end, none of thes seem to produce a consistent fix.

The version of ImageJ that is doing this was downloaded from MacBiophotonics
site.  I have installed and reinstalled this to no avail.  Interesting, I
have an older imageJ on the same machine that will open the same Image Stack
completely normally even as the MacBiophotonics 1.43b version is stuggling.

Of course this could be a pure Vista or Video driver problem, but I am at a
loss as to how to debug the problem.   Any suggestions or ideas why this is
happening?

Thanks

Rob

---------------------
Robert W. Baer, Ph.D.
Professor of Physiology
Kirksville College of Osteopathic Medicne
A. T. Still University of Health Sciences
800 W. Jefferson St.
Kirksville, MO 63501