Attachmentpoint-problem

Posted by maringa on Jul 08, 2009; 7:29am
URL: http://imagej.273.s1.nabble.com/Attachmentpoint-problem-tp3691862.html

Hi,

I posted a question a couple of weeks ago, about how to detect and count the attachmentpoints
of a soma (where the neurites attach to the soma) I got the advise to skeletonize two images, and
dilate or erode one of them with 1px and then subtract them from eachother. This just generates a lot of dots. I skeletonize, then set "count" in Binary Options to 6 and erode. Which should leave only the crossings. I also combined this by dilating with 'count=1' and 'iterations' a bit more than the line thickness.  
Then run 'Find Maxima' to get single points or count the patches (former crossings).

However, the result is this:
http://img193.imageshack.us/img193/7715/neurph4.jpg
just a lot of small dots...

Does anyone have another sugestion? It's kind of annoying to be stuck whit this simple (?) problem...

Thanks in advance :)