subcounting objects

Posted by Paolo Ariano on Jun 18, 2009; 10:39am
URL: http://imagej.273.s1.nabble.com/subcounting-objects-tp3692066.html

Hi all,

i'm trying to count spots in every cells ... so i've two pictures of the
same field of view:

1. the first picture shows the whole cells, so treshold and then analyze
particles and i obtain all the outlines saved and added to the
roi-manager

2. then i open the second image containing a lot of little spots inside
every cells area, now i'd like to count the spots number in every roi
i've in the roi-manager, how to finish the work ?

thanks a lot
paolo
--
NeuroScience PhD
Post-doc @ University of Torino
www.personalweb.unito.it/paolo.ariano
www.cellular-neurophysiology.unito.it
www.mieleria.it