Re: subcounting objects

Posted by Gabriel Landini on Jun 18, 2009; 11:31am
URL: http://imagej.273.s1.nabble.com/subcounting-objects-tp3692066p3692070.html

On Thursday 18 June 2009  12:27:51 Paolo Ariano wrote:

> i'm trying to count spots in every cells ... so i've two pictures of the
> same field of view:
>
> 1. the first picture shows the whole cells, so treshold and then analyze
> particles and i obtain all the outlines saved and added to the
> roi-manager
>
> 2. then i open the second image containing a lot of little spots inside
> every cells area, now i'd like to count the spots number in every roi
> i've in the roi-manager, how to finish the work ?

If you have the cells as binary objects and the particles to be counted as
"holes" then you can use the ParticleHoleNumber.txt macro in the morphology
collection.

Cheers,

G.