Calibrating density and creating calibrated image

Posted by Diana-3 on
URL: http://imagej.273.s1.nabble.com/Calibrating-density-and-creating-calibrated-image-tp3692091.html

I am working with autoradiography images - these are grey scale and need to
be calibrated to uCi using images of radioactive standards. I know how to
make and apply calibrations using the calibrate function and a rodbard curve.

My question: is it possible to CONVERT the original image (grey scale) into
a calibrated image where each pixel is converted into the new (calibrated)
value. In other words, i want to create and save this new calibrated image
as a map of uCi.

I read that if one saves calibrate image as tiff, the calibration curve will
be saved with it - this means next time i open this image in ImageJ and
perform measurements, it will measure uCi and not grey scale. But..., i want
to do region of interest analysis in another software which is why i need
saved images with calibrated pixels. I would be very keen to find out if
this is possible - perhaps someone out there has made an add-on that can do
this??

To explain - the reason i want to perform ROI analysis in another software
is because i want to save the ROI's - not just numbers but the actual
coordinates, which can be loaded into the image later, so that we can
sinspect and edit ROI's if necessary... I'm under impression that ImageJ
cannot do this but if i'm wrong please let me know!


Many thanks,
Diana