Re: Calibrating density and creating calibrated image

Posted by Michael Schmid on
URL: http://imagej.273.s1.nabble.com/Calibrating-density-and-creating-calibrated-image-tp3692091p3692092.html

Hi Diana,

you could also convert your calibrated image to 32 bit; then make  
sure that Edit>Options>Conversions> 'Scale when converting' is  
deselected and transform it back to 16-bit (or 8 bits, if your  
analysis software cannot read 16-bit tiffs).

Of course, you can have only integer values after the conversion, and  
the values will be between 0 and 65535 (16-bit) or 0-255 (8-bit).

Michael
________________________________________________________________

On 17 Jun 2009, at 19:06, Diana wrote:

> I am working with autoradiography images - these are grey scale and  
> need to
> be calibrated to uCi using images of radioactive standards. I know  
> how to
> make and apply calibrations using the calibrate function and a  
> rodbard curve.
>
> My question: is it possible to CONVERT the original image (grey  
> scale) into
> a calibrated image where each pixel is converted into the new  
> (calibrated)
> value. In other words, i want to create and save this new  
> calibrated image
> as a map of uCi.
>
> I read that if one saves calibrate image as tiff, the calibration  
> curve will
> be saved with it - this means next time i open this image in ImageJ  
> and
> perform measurements, it will measure uCi and not grey scale.  
> But..., i want
> to do region of interest analysis in another software which is why  
> i need
> saved images with calibrated pixels. I would be very keen to find  
> out if
> this is possible - perhaps someone out there has made an add-on  
> that can do
> this??
>
> To explain - the reason i want to perform ROI analysis in another  
> software
> is because i want to save the ROI's - not just numbers but the actual
> coordinates, which can be loaded into the image later, so that we can
> sinspect and edit ROI's if necessary... I'm under impression that  
> ImageJ
> cannot do this but if i'm wrong please let me know!
>
>
> Many thanks,
> Diana