Re: Calibrating density and creating calibrated image

Posted by Michael A. Miller on
URL: http://imagej.273.s1.nabble.com/Calibrating-density-and-creating-calibrated-image-tp3692091p3692094.html

>>>>> "Diana" == Diana  <[hidden email]> writes:

    > I am working with autoradiography images - these are grey
    > scale and need to be calibrated to uCi using images of
    > radioactive standards. I know how to make and apply
    > calibrations using the calibrate function and a rodbard
    > curve.

    > My question: is it possible to CONVERT the original image
    > (grey scale) into a calibrated image where each pixel is
    > converted into the new (calibrated) value. In other words,
    > i want to create and save this new calibrated image as a
    > map of uCi.


You can apply your calibration with the image math functions
available in the Process-Math->... menu.  If the original image
is integer data, make sure to convert the image to 32 bit
floating point values first (using Image->Type->32 bit).

If you save the image as raw values (or tiff), it can be loaded
into another program.

Mike


--
Michael A. Miller                             [hidden email]
  Department of Radiology, Indiana University School of Medicine