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Re: Attachmentpoints and Endpoint-problem

Posted by David Webster on Jun 15, 2009; 4:54am
URL: http://imagej.273.s1.nabble.com/Attachmentpoints-and-Endpoint-problem-tp3692095p3692099.html

You might want to look at Fiji's AnalyzeSkeleton plugin.

David Webster

On Sun, Jun 14, 2009 at 7:31 PM, maringa <[hidden email]>wrote:

> Hi there,
>
> I'm working with neurons and right now I'm stuck on one and a half problem.
> 1) Endpoint-detection of the neurites, working so so... (image)
> http://img191.imageshack.us/img191/433/endpoints.jpg
>
> My idea was to first remove the cellbodies, leaving the image with just
> neurites, and
> then erode by 1px (the neurites gets 1px shorter) and then skeletonize.
> This
> image
> is then subtracted from a copy of this skeletonized image, but the copy is
> not eroded
> so the subtraction results in small dots which I hoped to be the endpoints.
> Turned out to be a lot more irrellevant dots than I wanted...
>
> 2) Attachmentpoints.
>
> My idea here was to use dilate-filter, dilate the neurites by a couple of
> pixels to generate a cross
> where it enters the cellbody. Then use some kind of transformation to count
> the crosses... is this possible in the first place?
>
> There are a lot of problems associated with this, for example it's really
> hard to make a nice cross...
>
> Any ideas on this? It really got me stuck for a couple of weeks...
> --
> View this message in context:
> http://n2.nabble.com/Attachmentpoints-and-Endpoint-problem-tp3078257p3078257.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>