Re: remove noise ( Iterative deconvolution)

Posted by Runa Hamid on
URL: http://imagej.273.s1.nabble.com/Re-remove-noise-Iterative-deconvolution-tp3692100.html

Dear Zhengyu,
Thank you for the suggestions!

There is a plugin ' Iterative deconvolution' available in image J. Can I use
it for deconvoluting my time lapse images? I am slightly confused how do I
decide upon the last four options in this window.

 Runa


On Mon, Jun 15, 2009 at 9:39 PM, Pang, Zhengyu (GE, Research) <
[hidden email]> wrote:

> Runa,
>
> I don't know what you mean by photometircaly accurate. There are quite
> many factors will affect your final pixel intensity. Potentially it is
> hard to get to know how many photons getting to each pixel. If you want
> to remove noise that is arising from neighboring pixels, then what you
> need to do is to do a deconvolution. You need to know the point spread
> function (PSF) of your optical system. You need to purchase some
> sub-micron beads to measure it. Confocal microscope should have a small
> PSF compared to traditional epifluorescence microscope.
>
> I coauthored with Lavender at Biomaterials, and I did the experiment on
> point spread function measurement. If you are interested you should be
> able to google search it.
>
> Best regards,
>
> Zhengyu PANG
>
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Runa Hamid
> Sent: Monday, June 15, 2009 7:46 AM
> To: [hidden email]
> Subject: remove noise
>
> Dear Image J users,
>
> I have intensity values for each pixel in a given ROI in a time lapse
> series. I need to do some quantitation but before that I want to make it
> photometrically accurate. In other words I want to remove all noise that
> is arising in each pixel from neighbouring pixels, optical defects or
> electronic noises of a confocal system.
>
> I would be grateful if anyone can give me some useful tips/ suggestions
> to do it.
>
> Thanks in advance,
> Runa
>