Posted by
Pang, Zhengyu (GE Global Research) on
URL: http://imagej.273.s1.nabble.com/Re-remove-noise-Iterative-deconvolution-tp3692100p3692101.html
Runa,
I suppose that you should be able to use it. But I believe that
iterative deconvulution is a blind deconvolution method. It will do you
better if you read a few papers to understand this method and decide how
you want to use it. In addition, you can always ask the authors who
wrote this plugin for more information.
Best,
Zhengyu
Dr. Zhengyu Pang
Biochemistry and Biological Engineering Laboratory
General Electric Company Global Research
GE Imagination at work
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Runa Hamid
Sent: Wednesday, June 17, 2009 1:58 AM
To:
[hidden email]
Subject: Re: remove noise ( Iterative deconvolution)
Dear Zhengyu,
Thank you for the suggestions!
There is a plugin ' Iterative deconvolution' available in image J. Can I
use it for deconvoluting my time lapse images? I am slightly confused
how do I decide upon the last four options in this window.
Runa
On Mon, Jun 15, 2009 at 9:39 PM, Pang, Zhengyu (GE, Research) <
[hidden email]> wrote:
> Runa,
>
> I don't know what you mean by photometircaly accurate. There are quite
> many factors will affect your final pixel intensity. Potentially it is
> hard to get to know how many photons getting to each pixel. If you
> want to remove noise that is arising from neighboring pixels, then
> what you need to do is to do a deconvolution. You need to know the
> point spread function (PSF) of your optical system. You need to
> purchase some sub-micron beads to measure it. Confocal microscope
> should have a small PSF compared to traditional epifluorescence
microscope.
>
> I coauthored with Lavender at Biomaterials, and I did the experiment
> on point spread function measurement. If you are interested you should
> be able to google search it.
>
> Best regards,
>
> Zhengyu PANG
>
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> Runa Hamid
> Sent: Monday, June 15, 2009 7:46 AM
> To:
[hidden email]
> Subject: remove noise
>
> Dear Image J users,
>
> I have intensity values for each pixel in a given ROI in a time lapse
> series. I need to do some quantitation but before that I want to make
> it photometrically accurate. In other words I want to remove all noise
> that is arising in each pixel from neighbouring pixels, optical
> defects or electronic noises of a confocal system.
>
> I would be grateful if anyone can give me some useful tips/
> suggestions to do it.
>
> Thanks in advance,
> Runa
>