Re: Trichrome Stain Analysis

Posted by Francis Burton on Jun 16, 2009; 9:54pm
URL: http://imagej.273.s1.nabble.com/Trichrome-Stain-Analysis-tp3692117p3692118.html

Hello Dayana,

>After staining with Masson's Trichrome stain, I am analyzing
>immunohistochemistry images for the amount of blue collagen vs. red muscle
>fibers.  I am having trouble finding a plug-in the can help me distinguish
>and count the blue vs. red areas in the images.  Could anyone help me?

Trichrome segmentation is an almost archetypal application of k-means
clustering. There is an ImageJ plugin that implements this at:

http://ij-plugins.sourceforge.net/plugins/clustering/index.html

It is used as an example in this paper:

http://www.biotechniques.com/biotechniques/BiotechniquesJournal/supplements/2007/July/ImageJ-for-microscopy/biotechniques-42894.html?pageNum=2

Hope that helps!

Francis