Re: Trichrome Stain Analysis

Posted by David Webster on Jun 16, 2009; 8:35pm
URL: http://imagej.273.s1.nabble.com/Trichrome-Stain-Analysis-tp3692117p3692119.html

Dayana,

Try G. Landini's *Threshold_Colour*  plugin. You can get to it via
ImageJ/Plugins. Then look up Landini's page. The plugin will compute HSV
histogrms from an RGB image. I found that varying the threshold on the Hue
histogram separates out color regions in the image nicely. This should be a
good start point.

David Webster

On Tue, Jun 16, 2009 at 10:32 AM, Dayana Webster <[hidden email]> wrote:

> Hello,
> After staining with Masson's Trichrome stain, I am analyzing
> immunohistochemistry images for the amount of blue collagen vs. red muscle
> fibers.  I am having trouble finding a plug-in the can help me distinguish
> and count the blue vs. red areas in the images.  Could anyone help me?
>
> Thanks,
> Dayana Webster
>