calibrate time-series image photobleaching in ImageJ

Posted by Liu, Dongfang (NIH/NIAID) [F] on Jun 01, 2009; 6:07am
URL: http://imagej.273.s1.nabble.com/calibrate-time-series-image-photobleaching-in-ImageJ-tp3692361.html

Hi All,

I have time-series image which tracks the intensity as a function of time. There is photobleaching in my time-series image stack. Because I tracked the intensity of non-signal region, there is obvious decay of intensity. I think such photobleaching must affect the intensity of signal region. Do you know how to correct photobleaching in ImageJ? In other words, how to avoid my signals are affected by photobleaching? How to calibrate it?

Thanks.
Don