Re: calibrate time-series image photobleaching in ImageJ
Posted by
lechristophe on
Jun 01, 2009; 6:54am
URL: http://imagej.273.s1.nabble.com/calibrate-time-series-image-photobleaching-in-ImageJ-tp3692361p3692362.html
Have a look here :
http://www.macbiophotonics.ca/imagej/t.htm#t_bleachIn the MBF distribution of ImageJ, there is a plugin that is using an
exponentional decay to model bleach. There is also a macro from Jens
Rietdorf, however the link seems to be broken :
http://www.embl-heidelberg.de/ExternalInfo/almf/htdocs/almf_website/html/downloads.htmlChristophe
On Mon, Jun 1, 2009 at 8:07 AM, Liu, Dongfang (NIH/NIAID) [F] <
[hidden email]> wrote:
> Hi All,
>
> I have time-series image which tracks the intensity as a function of time.
> There is photobleaching in my time-series image stack. Because I tracked the
> intensity of non-signal region, there is obvious decay of intensity. I think
> such photobleaching must affect the intensity of signal region. Do you know
> how to correct photobleaching in ImageJ? In other words, how to avoid my
> signals are affected by photobleaching? How to calibrate it?
>
> Thanks.
> Don
>