Re: Paste and Blend only overlapping regions (MakeMontage variation)

Posted by Daniel James White on May 26, 2009; 8:14am
URL: http://imagej.273.s1.nabble.com/Paste-and-Blend-only-overlapping-regions-MakeMontage-variation-tp3692412p3692413.html

On May 26, 2009, at 6:00 AM, IMAGEJ automatic digest system wrote:

> Date:    Mon, 25 May 2009 04:55:16 -0400
> From:    Bruno Keijzers <[hidden email]>
> Subject: Re: Paste and Blend only overlapping regions (MakeMontage  
> variation)
>
> On Sat, 23 May 2009 11:05:20 +0200, Alan Hewat=20
> <[hidden email]> wrote:
>
>> I have looked at various montage macros for creating composite  
>> mosaics
>> from overlapping images, and wanted to try something simpler using
>> "imageJ/Stacks/Make Montage". Since my image offsets required for
>> registration are constant, I can modify the MakeMontage.txt macro to
>> offset the image rectangles before pasting.
>
> I made a macro that converts a stack of images with an equal  
> vertical=20
> offset into a single image. The overlapping parts are blended. Maybe  
> this=
> =20
> helps.
>
> // This macro stiches images from a stack into a new image
> //works only for 32 bit images (convert stack to 32 bit imege first).
> // displacement+overlap defines distance in pixels between two images.
> // overlap is the area that is blended via a wedge shape.
>
> macro "Stich Stack" { .....

Thats nice,
but what if your image offsets are not exactly (eg plus minus 1 pixel)  
correct ?
Can they really be that good on a real optical system?

I suspect that
Stitch Directory with Images (unknown configuration)
from Fiji  (Fiji is just imageJ - batteries included)
http://pacific.mpi-cbg.de/wiki/index.php/Stitching

will give a better result,
as it relies on the information in the overlapping parts of the image,
rather than your best estimate of the real overlap.

You dont even need to tell it the tile layout, as it figures it out  
itself,
and it does some kind of global optimisation where 4 images overlap,
so you get the best result there,
and has nice blending options....

Dan






Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
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01307 DRESDEN
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