Re: anisotropy image

Posted by Michael Elbaum on
URL: http://imagej.273.s1.nabble.com/anisotropy-image-tp3692489p3692490.html

Hi.    You should convert the images to 32 bit format, which holds fractional values.
Michael


>>> Virginia Borroni <[hidden email]> 05/15/09 9:51 PM >>>
We do it by homoFRET on cells.
Quoting Michael Doube <[hidden email]>:

> Hi Virginia
>
> What do you mean by "anisotropy image"?
>
> There are a few ways to calculate anisotropy, including star volume,
> mean intercept length and autocorrelation.  Is this what you want to do?
>
> Mike
>
> Virginia Borroni wrote:
>> Hi,
>> I would like to know if Imagej can be used to generate an anisotropy image.
>> I have tryed but when I do the math with the images in both channels
>> (paralel and perpendicular) I only get a black image in wich every pixel is
>> 0. I know that is because I get negative and fractional numbers, but how can
>> I overcome this problem? Thanks a lot
>> Virginia Borroni

Maria Virginia Borroni
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