Re: anisotropy image

Posted by Virginia Borroni on
URL: http://imagej.273.s1.nabble.com/anisotropy-image-tp3692489p3692491.html

Thanks a lot, I will try that!
Virginia


Quoting Michael Elbaum <[hidden email]>:

> Hi.    You should convert the images to 32 bit format, which holds  
> fractional values.
> Michael
>
>
>>>> Virginia Borroni <[hidden email]> 05/15/09 9:51 PM >>>
> We do it by homoFRET on cells.
> Quoting Michael Doube <[hidden email]>:
>
>> Hi Virginia
>>
>> What do you mean by "anisotropy image"?
>>
>> There are a few ways to calculate anisotropy, including star volume,
>> mean intercept length and autocorrelation.  Is this what you want to do?
>>
>> Mike
>>
>> Virginia Borroni wrote:
>>> Hi,
>>> I would like to know if Imagej can be used to generate an anisotropy image.
>>> I have tryed but when I do the math with the images in both channels
>>> (paralel and perpendicular) I only get a black image in wich every pixel is
>>> 0. I know that is because I get negative and fractional numbers,  
>>> but how can
>>> I overcome this problem? Thanks a lot
>>> Virginia Borroni
>
>
>
> Maria Virginia Borroni
> INIBIBB
> C La Carrindanga Km 7 CC857. B8000FWB.
> Bahia Blanca. Buenos Aires. Argentina.
> Tel.#: [phone]. interno 127
> Fax#: [phone]
> [hidden email]
>
>
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Maria Virginia Borroni
INIBIBB
C La Carrindanga Km 7 CC857. B8000FWB.
Bahia Blanca. Buenos Aires. Argentina.
Tel.#: [phone]. interno 127
Fax#: [phone]
[hidden email]


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