Our fluorescence images are usually composed of some spread spots, and local weighted mean (lwm) is the best option. And there is no this transform in TurboReg/StackReg plugins. I hope to get more help on this from everyone. Thanks.
> Date: Thu, 14 May 2009 10:32:51 -0400
> From:
[hidden email]
> Subject: Re: Image alignment
> To:
[hidden email]
>
> The TurboReg/StackReg plugins are very good. We use them with our plugin.
>
>
http://bigwww.epfl.ch/thevenaz/turboreg/>
> Shaoqing Zhang wrote:
> > Dear everyone,
> >
> > Now I am doing FRET image analysis. And I am now using Matlab command
> >
> > tform = cp2tform(input_points, base_points, 'lwm')
> >
> > to align the CFP and YFP images.
> >
> > But it is required a lot of reference points which are obtained manually and usually I have to many images to deal with. Is there a better (automated at best) way to do image registration? Thank you very much in advance.
> >
> > Sincerely,
> >
> > Shaoqing Zhang
> > University of Pennsylvania
> >
>
>
>
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