Re: MTrack2

Posted by Aryeh Weiss on May 01, 2009; 3:57am
URL: http://imagej.273.s1.nabble.com/MTrack2-tp3692712p3692714.html

Gabriel Landini wrote:

>> I tried setting white as foreground and black as background in
>> edit>options>colors...  but that does not seem to do it.
>>
> aryeh:
> I also use white particles on a black background by default for all the things
> I do, but never used MTrack2.
>
> Have a look into this ridiculously long titled link:
>
> http://imagejdocu.tudor.lu/doku.php?id=faq:technical:how_do_i_set_up_imagej_to_deal_with_white_particles_on_a_black_background_by_default
>
> You perhaps need to also select the binary options (explained in the page
> above).
> I hope it helps,
>
> Gabriel
>
>

Thank you for your quick reply. I tried this -- it appears to be equivalent
to selecting the dark background checkbox during apply threshold.
However, MTrack2 still fails in this case.

The interesting thing is that if I use the black background option, then MTrack2
fails, but if I then invert the LUT, them MTrack2 works. One would think that
the LUT should play no role, because the image values do not change.

Essentially, I can have two images that are identical, except that one displays
255 and black and 0 as white, while one displays 255 as white and 0 as black,
and get two different results. That is strange.

--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

Israel Society for Microscopy 2009 meeting website:
http://materials.technion.ac.il/ism/ISM2009.html