Posted by
AJBell on
Apr 27, 2009; 9:07am
URL: http://imagej.273.s1.nabble.com/Re-Florescence-Intensity-tp3692762.html
Sam,
Speaking from personal experience, I can honestly say it may not be
possible due to small variations between the images. That being said, the
method I used to calculate intensity per cell was to threshold the image
automatically, create selection, analyse particles then take measurements
of cell size and Integrated density.
This can be put into a macro quite easily.
Hope that helps.
Andrew
On Fri, 10 Apr 2009 14:11:46 +0100, Sam Murray
<
[hidden email]> wrote:
>Hello. I was wondering whether someone could help me please. I am
looking at the effect of growing brain cells in 3 different sera (human,
serum free and fetal calf). I would like to see the effect of the sera on
8 different antibodies by flow cytometry and immunocytochemistry. The flow
cytometry analysis is complete, I would now like to compare the ICC. All
micrographs have been taken with a b&w camera and all with the same
settings. How can I use ImageJ to quantify the fluorescence intensity? I
probably wouldn't need to subtract the background as I have appropriate
background ICCs. I have around 200 micrographs, so some sort of
automation/plugin would be good.
>
>Your help is very much appreciated.
>Kind regards,
>Sam
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