Reslice from line selection...

Posted by Michael Cundell on Apr 24, 2009; 4:54pm
URL: http://imagej.273.s1.nabble.com/Reslice-from-line-selection-tp3692795.html

Hello.

Im sorry I have another question about reslice.  When using the reslice tool with a line selection and the slice count set to lets say 10, in the image the line becomes a rectangular box.  I was presuming that box defined the boundaries of the reslice, as is the case when you make a rectangular selection.  However, when i start the reslice the lines go beyond that box.

Im working with a deltavision file.  I am still unsure of what I should be setting the input and output spacing at for a 1:1 representation of my image data that is biologically relevent but I dont want to hassle the list about this but did want one last attempt to try and find an answer (sorry !).  I just chose 0.11 micron input and output spacing as this is the width of a single pixel as measured by the deltavision microscopes own software and also when selecting a single pixel with the rectangular selection in imageJ the h and w values are 0.11.  However, changing this input and output spacing also effects how far beyond the box the lines go when reslicing from a line selection so it may be relevent to this question.

Basically I have 20 line ROIs selected in an image and want to make a macro to reslice them and create max intensity z-projections.  I just need to make sure that in the macro i set the slice count to something that will cover the region im interested in and not go too far beyond that or it will start reslicing other cells and im trying to slice something from a single cell.

Any help would be appreciated.

Thanks !

Mike