Mask and measure

Posted by AJBell on Apr 20, 2009; 11:05am
URL: http://imagej.273.s1.nabble.com/Mask-and-measure-tp3692815.html

Firstly if this has come up before, please accept my appologies, but I
could not find the answer on the list database.

For each FOV I have a phase and fluorescence image. What I want to be able
to do is to calculate the fluorescence per unit area of each object in the
FOV.

More precisely, I am trying to calculate the fluorescence per unit area
based upon the area obtained in the phase image (In effect, I have an
object that is sized in phase images, but is not all sizeable in
fluorescent images).

To do this, the obvious thing would be to create a mask of the phase image
and then use this to identify the ROI in the fluorescence images. However,
although I can make the mask, I can't seem to use it as the ROI for the
fluorescence images.

Is this even possible?

(Did that make sense to anyone?)

Thanks in advance

Andrew