Try to split mask in Roi manager and then use individual selection for measuring fluorescence on second image
>-----Original Message-----
>From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of AJ
>Bell
>Sent: 20 April 2009 12:06
>To:
[hidden email]
>Subject: Mask and measure
>
>Firstly if this has come up before, please accept my appologies, but I
>could not find the answer on the list database.
>
>For each FOV I have a phase and fluorescence image. What I want to be
>able
>to do is to calculate the fluorescence per unit area of each object in
>the
>FOV.
>
>More precisely, I am trying to calculate the fluorescence per unit area
>based upon the area obtained in the phase image (In effect, I have an
>object that is sized in phase images, but is not all sizeable in
>fluorescent images).
>
>To do this, the obvious thing would be to create a mask of the phase
>image
>and then use this to identify the ROI in the fluorescence images.
>However,
>although I can make the mask, I can't seem to use it as the ROI for the
>fluorescence images.
>
>Is this even possible?
>
>(Did that make sense to anyone?)
>
>Thanks in advance
>
>Andrew