Fluorescence Intensity

Posted by Sam Angel on Jan 16, 2009; 3:17pm
URL: http://imagej.273.s1.nabble.com/Fluorescence-Intensity-tp3692824.html

Hello.  I was wondering whether someone could help me please. I am running four different experiments which I hope will correlate at the end of the day. The basis is the same, I am looking at the effect of growing brain cells (1 normal brain cell line, 1 brain tumour (high passage), and 2 brain tumours (low passage)) in 3 different sera (human, serum free and fetal calf). For two of the experiments (flow cytometry and immunocytochemistry), the cells are then exposed to 8 different antibodies. With Flow Cytometry, the geometric mean fluorescence intensity shows that there is a trend in both cell type and the sera in which the cells are grown. What I would like now is to do some stats with the ICC. I have today discovered ImageJ, but cannot find appropriate instructions. Unfortunately, my micrographs haven't been taken in a standard fashion, (number of cells in the field of view, exposure, brightness, contrast, etc); whereas my Flow data was all captured with one standard set of the instrumentation. Will it be possible to standardise my images in order to compare them? How best would I get some data? If at all possible?

Your help is very much appreciated.
Kind regards,
Sam