Re: Fluorescence Intensity

Posted by Sam Angel on Apr 21, 2009; 10:14pm
URL: http://imagej.273.s1.nabble.com/Fluorescence-Intensity-tp3692824p3692825.html

Sam Angel wrote

Hello.  I was wondering whether someone could help me please. I am looking at the effect of growing brain cells in 3 different sera (human, serum free and fetal calf). I would like to see the effect of the sera on 8 different antibodies by flow cytometry and immunocytochemistry. The flow cytometry analysis is complete, I would now like to compare the ICC. I have now standardised my ICC micrographs and was wondering how I can use ImageJ to quantify the fluorescence intensity? I probably wouldn't need to subtract the background as I have appropriate background ICCs. I have around 200 micrographs, so some sort of automation/plugin would be good.

Your help is very much appreciated.
Kind regards,
Sam